Crossed Immunoelectrophoresis and Electroimmunoassay of IgM
Open Access
- 1 April 1974
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 112 (4) , 1420-1425
- https://doi.org/10.4049/jimmunol.112.4.1420
Abstract
Analysis of IgM by crossed immunoelectrophoresis is possible if the original technique is modified by the use of: agarose gel with little, or preferably no, electroendosmosis; a high pH of 10.3; and an antibody population essentially isoelectric at pH 10.3. Such an antibody population can easily be produced by a chemical modification, under mild conditions, of about one-third of the carboxylic acid groups of the antibodies. This analytical system also permits rapid quantitation of IgM by the electroimmunoassay.Keywords
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