The design of syntetic genes
Open Access
- 1 January 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 16 (5) , 1693-1702
- https://doi.org/10.1093/nar/16.5.1693
Abstract
Computer programs are described that aid in the design of synthetic genes coding for proteins that are targets of a research program in site directed mutagenesis. These programs “reverse-translate” protein sequences into general nucleic acid sequences (those where codons have not yet been selected), map restriction sites into general DNA sequences, identify points in the synthetic gene where unique restriction sites can be introduced, and assist in the design of genes coding for hybrids and evolutionary intermediates between homologous pro teins. Application of these programs therefore facilitates the use of modular mutagenesis to create variants of proteins, and the implementation of evolutionary guidance as a strategy for selecting mutants.This publication has 19 references indexed in Scilit:
- Tinkering with Enzymes: What Are We Learning?Science, 1987
- Synthesis, bacterial expression, and mutagenesis of the gene coding for mammalian cytochrome b5.Proceedings of the National Academy of Sciences, 1986
- Cloning and Expression in Escherichia coli of a Synthetic DNA for Hirudin, the Blood Coagulation Inhibitor in the LeechDNA, 1986
- EFFECTS OF SITE-SPECIFIC AMINO ACID MODIFICATION ON PROTEIN INTERACTIONS AND BIOLOGICAL FUNCTIONAnnual Review of Biochemistry, 1985
- Redesigning Trypsin: Alteration of Substrate SpecificityScience, 1985
- Synthesis of a gene for human growth hormone and its expression in Escherichia coli.Proceedings of the National Academy of Sciences, 1984
- Total Synthesis and Cloning of a Gene Coding for the Ribonuclease S ProteinScience, 1984
- Total synthesis of a human leukocyte interferon geneNature, 1981
- Chemical synthesis of genes for human insulin.Proceedings of the National Academy of Sciences, 1978
- Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone SomatostatinScience, 1977