Identification and purification of the Lon + (capR + ) gene product, a DNA-binding protein
- 1 April 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (4) , 2043-2047
- https://doi.org/10.1073/pnas.78.4.2043
Abstract
The polypeptide product of the lon ( capR ) gene was identified and partially purified from bacterial strains homozygous for the capR + or capR9 (ochre mutation) alleles cloned with pSC101. A 94,000-dalton polypeptide was identified as the lon (capR) gene product. Studies of binding to DNA cellulose columns and nitrocellulose filters indicate that the capR + and capR9 proteins bind DNA.Keywords
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