Sampling of Nicotiana tabacum leaf lamina to surmount the problem of nonuniform distribution of total alkaloids

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Abstract
Nonuniform distribution of total alkaloids in leaf lamina of Nicotiana tabacum requires precise positioning of the fixed diameter cutter in obtaining leaf disks which provide a reliable estimate of total alkaloids content of the leaf. This is time-consuming. Other methods such as the whole leaf technique yield excessive amounts of tissue to process. Our procedure uses a band technique that overcomes the problems associated with the unequal distribution of total alkaloids in the lamina and increases sampling rate threefold. This procedure is superior to others for sampling the different sizes of leaves on the plant at anyone time until maturity.

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