Elemental microanalysis of individual blood cells
- 1 August 1987
- journal article
- Published by Springer Nature in Biological Trace Element Research
- Vol. 13 (1) , 159-166
- https://doi.org/10.1007/bf02796629
Abstract
A scanning proton microprobe was used to study single red blood cells in freeze-dried, whole-blood specimens. Simultaneous collection of PIXE and of forward and backward scattered proton data provided information on the heavier elements (Z>27) and on the organic mass under investigation. The trace elemental spectrum of a red cell was found to be reproducible, and no elemental losses were observed during proton bombardment. The spatial resolution of the probe (1.5 μm for these studies) enabled the red cell’s biconcave disk shape to be visualized in quantitative two- and three-dimensional maps of H, C, P, S, Cl, K, and Fe.Keywords
This publication has 8 references indexed in Scilit:
- Developments on the Melbourne scanning proton microprobePublished by Elsevier ,2002
- QUANTITATIVE MICROANALYSIS OF MN, ZN AND OTHER ELEMENTS IN MATURE WHEAT SEEDNew Phytologist, 1985
- Application of the nuclear microprobe to the study of elemental profiles in individual blood cells. Preparation and analysisNuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 1984
- Elemental Analyses Performed with the Karlsruhe Nuclear MicroprobeIEEE Transactions on Nuclear Science, 1983
- Application of a scanning proton microprobe to biology and medicineNuclear Instruments and Methods in Physics Research, 1982
- The distribution of trace elements in mature wheat seed using the Melbourne proton microprobeNuclear Instruments and Methods in Physics Research, 1981
- Total quantitative recording of elemental maps and spectra with a scanning microprobeJournal of Microscopy, 1979
- The Melbourne proton microprobeJournal of Microscopy, 1979