Fermenter Growth of Streptococcus agalactiae and Large-scale Production of CAMP Factor

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Abstract
S. agalactiae (group B) was grown in Todd-Hewitt broth (36.4 g l-1, pH 7.8) in a Braun Fermenter (type B20) to investigate the conditions of optimal bacterial growth and maximal production of CAMP [Christie-Atkins-Munch-Petersen] factor. The influence of different gas atmospheres (air, N2, CO2 and gas mixtures) on growth, CAMP production and chain length of S. agalactiae was studied. The organisms grew best in the presence of 2% (wt/vol) glucose, at pH 6.2, with a constant flow of CO2. The number of diplococci and monococci under these conditions reached almost 80% of the total population.