Indoxyl-tetranitro blue tetrazolium method for detection of alkaline phosphatase in immunohistochemistry.
Open Access
- 1 August 1989
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 37 (8) , 1299-1301
- https://doi.org/10.1177/37.8.2474024
Abstract
A sensitive method for detection of alkaline phosphatase in immunohistochemistry, using lymphoid cells, has been optimized. The conditions for staining are 0.23 mM 5-bromo-4-chloro-indoxyl phosphate, 0.55 mM tetranitro blue tetrazolium, 2.0 mM levamisole, 5.0 mM sodium azide, 10.0 mM magnesium chloride, and 0.15 mM 1-methoxyphenazine methosulfate dissolved in 100 mM Tris-HCl buffer, pH 9.5.This publication has 16 references indexed in Scilit:
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