Determination of Caffeine in Human Urine Samples Free of the Interference of its Metabolites by Reversed-Phase Liquid Chromatography Using Solid-Phase Extraction from Sample Clean-Up
- 1 April 1993
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography & Related Technologies
- Vol. 16 (6) , 1297-1314
- https://doi.org/10.1080/10826079308020954
Abstract
This study describes a rapid assay for caffeine in urine samples combining solid-phase extraction techniques for sample clean-up, and conventional reversed-phase conditions for elution. The samples are previously conditioned usin24Jul1992g C18 solid-phase extraction columns, and chromatographed on an HP-Hypersil ODS-C18 column with a mobile Phase consisting of acetoni tr i le-phosphate buffer (pH=3) in gradient elution mode. The detection is set at 275 nm. These conditions provide a complete resolution of caffrence in c. a. 6 min. The described system can oe applied to the simultaneous determination of caffeine and its main metabolites. The selectivity, sensitivit and precision at therapeutic levels make it suitable for caffeine monitoring.Keywords
This publication has 0 references indexed in Scilit: