Stable isotope probing — linking microbial identity to function
Top Cited Papers
- 10 May 2005
- journal article
- review article
- Published by Springer Nature in Nature Reviews Microbiology
- Vol. 3 (6) , 499-504
- https://doi.org/10.1038/nrmicro1162
Abstract
Stable isotope probing (SIP) is a technique that is used to identify the microorganisms in environmental samples that use a particular growth substrate. The method relies on the incorporation of a substrate that is highly enriched in a stable isotope, such as (13)C, and the identification of active microorganisms by the selective recovery and analysis of isotope-enriched cellular components. DNA and rRNA are the most informative taxonomic biomarkers and (13)C-labelled molecules can be purified from unlabelled nucleic acid by density-gradient centrifugation. The future holds great promise for SIP, particularly when combined with other emerging technologies such as microarrays and metagenomics.Keywords
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