INDUCTION OF MALIGNANT TRANSFORMATION OF COCULTIVATED HEMATOPOIETIC STEM-CELLS BY X-IRRADIATION OF MURINE BONE-MARROW STROMAL CELLS-INVITRO
- 1 September 1986
- journal article
- research article
- Vol. 46 (9) , 4677-4684
Abstract
X-irradiation of purified primary cultures of mouse bone marrow stroma or permanent cloned marrow stromal cell lines in plateau phase decreases production of macrophage progenitor cell-specific colony-stimulating factor to a plateau minimum of 40% of control levels after doses of 50 to 500 Gy delivered at 2 Gy/min. After 50 Gy there is increased bioavailability of another growth factor(s) that is distinct from macrophage progenitor cell-specific colony-stimulating factor, granulocyte-macrophage progenitor cell colony-stimulating factor, or colony-stimulating factor for multipotential hematopoietic stem cells (interleukin 3). Liquid-phase cocultivation of irradiated stromal cells with either nonadherent cells from continuous marrow cultures or cloned dual granulocyte-macrophage progenitor cell colony-stimulating factor/interleukin 3-dependent hematopoietic progenitor cell lines induces evolution over 5 weeks of factor-independent colony-forming cells. Subcultured factor-independent colonies generated clonal malignant cell lines with multiple distinct karyotypic alterations. Inoculation of 106 cells s.c. from factor-independent clones into syngeneic mice produces local granulocytic monomyeloid tumors with spread to spleen, lymph nodes, and bone marrow. These data provide the first demonstration in vitro of indirect X-irradiation leukemogenesis through cells of the marrow stroma.This publication has 39 references indexed in Scilit:
- Cloned human and mouse kappa immunoglobulin constant and J region genes conserve homology in functional segmentsCell, 1980
- Growth of factor-dependent hemopoietic precursor cell lines.The Journal of Experimental Medicine, 1980
- Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.Proceedings of the National Academy of Sciences, 1980
- HEMOPATHOLOGIC CONSEQUENCES OF PROTRACTED GAMMA-IRRADIATION - ALTERATIONS IN GRANULOCYTE RESERVES AND GRANULOCYTE MOBILIZATION1980
- Self‐renewal of factor‐dependent hemopoietic progenitor cell‐lines derived from long‐term bone marrow cultures demonstrates significant mouse strain genotypic variationJournal of Supramolecular Structure, 1980
- Dose-Response Relationship of Neutrons and γ Rays to Leukemia Incidence among Atomic Bomb Survivors in Hiroshima and Nagasaki by Type of Leukemia, 1950-1971Radiation Research, 1979
- Levels of translatable mRNAs for cell surface protein, collagen precursors, and two membrane proteins are altered in Rous sarcoma virus-transformed chick embryo fibroblasts.Proceedings of the National Academy of Sciences, 1977
- Conditions controlling the proliferation of haemopoietic stem cells in vitroJournal of Cellular Physiology, 1977
- Biological and serological characterization of radiation leukemia virus.Proceedings of the National Academy of Sciences, 1976
- MYELOID LEUKEMIA IN THE MOUSEAnnals of the New York Academy of Sciences, 1964