Effect of Exogenous Long-Chain Fatty Acids on Lipid Biosynthesis in Dispersed Ruminant Mammary Gland Epithelial Cells: Esterification of Long-Chain Exogenous Fatty Acids

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Abstract
Dispersed epithelial cells from lactating bovine and goat mammary glands incorporated acetate into all fatty acids (C4 to C16) that were incorporated into mainly triacylglycerols. The cells secreted free fatty acids only into the incubation medium, and this secretion was dependent on the concentration of albumin and the type and amount of exogenous fatty acid added to the medium. Addition of palmitic acid to the incubation medium stimulated synthesis and incorporation of fatty acids synthesized de novo into triacylglycerols, whereas stearic and linoleic acid were inhibitory.