Germ line transmission of an inactive N-myc allele generated by homologous recombination in mouse embryonic stem cells.
Open Access
- 1 December 1990
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 10 (12) , 6755-6758
- https://doi.org/10.1128/mcb.10.12.6755
Abstract
We have disrupted one allele of the N-myc locus in mouse embryonic stem (ES) cells by using homologous recombination techniques and have obtained germ line transmission of null N-myc ES cell lines with transmission of the null N-myc allele to the offspring. The creation of mice with a deficient N-myc allele will allow the generation of offspring bearing null N-myc alleles in both chromosomes and permit study of the role that this proto-oncogene plays in embryonic development.This publication has 15 references indexed in Scilit:
- Towards a molecular-genetic analysis of mammalian developmentTrends in Genetics, 1989
- Germ-Line Transmission of a c- abl Mutation Produced by Targeted Gene Disruption in ES CellsScience, 1989
- Altering the Genome by Homologous RecombinationScience, 1989
- Contrasting patterns of myc and N-myc expression during gastrulation of the mouse embryo.Genes & Development, 1989
- Targetted correction of a mutant HPRT gene in mouse embryonic stem cellsNature, 1987
- Differential expression of myc family genes during murine developmentNature, 1986
- Expression of N-myc in teratocarcinoma stem cells and mouse embryosNature, 1985
- Activated Expression of the N- myc Gene in Human Neuroblastomas and Related TumorsScience, 1984
- Transposition and amplification of oncogene-related sequences in human neuroblastomasCell, 1983
- Amplified DNA with limited homology to myc cellular oncogene is shared by human neuroblastoma cell lines and a neuroblastoma tumourNature, 1983