High Performance Liquid Chromatography of Albendazole and Its Sulfoxide Metabolite in Human Organs and Fluids during Hydatatidosis

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Abstract
Albendazole and albendazole sulfoxide were quantified in serum, bile, liver, lungs and hydatid cyst fluid and walls of a man receiving albendazole before surgical procedure during therapy of hydatidosis. The frozen tissue specimens were crushed and suspended in phosphate buffer, then the suspension was extracted with ethylacetate as for liquid specimens . The parent drug and its metabolite were extracted at the same time. Albendazole was quantified on a bonded hydrophobic stationary phase and albendazole sulfoxide on a silica column at 254 nm. This assay was designed for monitoring albendazole and albendazole sulfoxide in serum of patients on long course treatment. The limit of sensitivity in serum was 10 ng/ml for the parent drug and its metabolite. An accumulation of these 2 drugs was shown in organs and bile, sometimes in the liquid and membrane of the cyst.