CELLULAR IMMUNITY SHOWN IN PSEUDORABIES VIRUS-INFECTED PIGS BY LEUKOCYTE MIGRATION-INHIBITION PROCEDURE

  • 1 January 1979
    • journal article
    • research article
    • Vol. 40  (1) , 66-68
Abstract
Cellular immunity in pigs inoculated with pseudorabies virus (PRV) was studied by the agarose plate technique of direct leukocyte migration-inhibition procedure. Migration of leukocytes from PRV-infected pigs was inhibited in the presence of PRV antigen [Ag], whereas migration of leukocytes from nonexposed pigs was not inhibited in the presence of the same Ag. The migration of leukocytes collected 4 days after intranasal exposure to PRV was inhibited; humoral antibodies [Ab] could not be detected until 7 days after exposure. Cellular immunity was present in pigs 14 days after inoculation with inactivated PRV Ag; low concentrations of neutralizing and precipitating Ab were present at this time. The leukocyte migration-inhibition procedure is a useful tool in studying the role of cellular immunity in PRV infections.