OH Radical-induced Products of Tyrosine Peptides

Abstract
Reactions of radiation-generated OH radicals with tyrosine and its homopeptides, i.e. L-Tyr-L-Tyr and L-Tyr-L-Tyr-L-Tyr, in N2O saturated solutions were shown to give crosslinks between the peptide chains with high yields. High-performance liquid chromatography, capillary gas chromatography and mass spectrometry were used for isolation and identification of the monomeric and dimeric products. Evidence is presented for the crosslinking to occur through C-C and C-O-C bonds. As far as is known, the formation of the ether type of crosslink is demonstrated for the 1st time. Mechanisms of product formation are also discussed, which involve radicals that were described in previous pulse radiolysis studies.

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