Interfering polysialyltransferase ST8SiaII/STX mRNA inhibits neurite growth during early hippocampal development
- 26 July 2006
- journal article
- Published by Wiley in FEBS Letters
- Vol. 580 (19) , 4723-4726
- https://doi.org/10.1016/j.febslet.2006.07.058
Abstract
Polysialic acid (PSA) attached to NCAM is involved in cell–cell interactions participating in structural and functional plasticity of neuronal circuits. Two polysialyltransferases, ST8SiaII/STX and ST8SiaIV/PST, polysialylate NCAM. We previously suggested that ST8SiaII/STX is the key enzyme for polysialylation in hippocampus. Here, polysialyltransferase mRNA interference experiments showed that, knock down of ST8SiaIV/PST transcripts did not affect PSA expression, but PSA was almost absent from neuronal surfaces when ST8SiaII/STX mRNA was interfered. Non-polysialylated neurons bore a similar number of neurites per cell than polysialylated neurons. However, non-polysialylated processes were shorter and a lower density of synaptophysin clusters accompanied this reduced neuritic growth. Therefore, ST8SiaII/STX expression is essential to allow a correct neuritic development at initial stages of hippocampus ontogenyKeywords
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