The mode of action of a novel 18-membered macrolide, virustomycin A (AM-2604A), on Trichomonas foetus.

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Abstract
The mode of action of virustomycin A, a novel 18-membered macrolide, on T. foetus was investigated. The antibiotic inhibited the biosynthesis of RNA, DNA and protein in the organism. The inhibition of RNA biosynthesis was the most severe. Virustomycin A repressed the incorporation of [3H]uridine into both acid-soluble and insoluble fractions, whereas actinomycin D inhibited the incorporation of [3H]uridine into acid-insoluble fraction alone. Virustomycin A interfered with nucleotide formation from uridine and adenosine but not with their transport to the cells. The antibiotic did not inhibit the activities of uridine kinase and uracil phosphoribosyltransferase in a cell-free extract from the organism. Probably, it interferes with the formation of phosphate donor(s) (possibly ATP-forming system) of the organism.

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