Studies on transfer ribonucleic acids and related compounds. VIII(1). Further studies on aromatic phosphoramidates as a protecting group for phosphomonoesters
- 1 February 1974
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 1 (2) , 223-234
- https://doi.org/10.1093/nar/1.2.223
Abstract
Stability of aromatic phosphoramidates was studied using 2′, 3′-O-di-benzoyluridine5′-phosphoramidates and N,2′,3′-O-tribenzoylcytidine 5′-phosphate. The effect of dicyclohexylcarbodiimide in this mixture was investigated. Deconposition of the anilidate was slower in the presence of DCC. Substituted anilidates of uridine 5′-phosphate were synthesized and the stability of these amidates in anhydrous pyridine was studied. 2′-O-Benzoyluridine 3′-phosphoranilidate and the corresponding β-naphthylidatewere conpared in their stabilities in anhydrous pyridine, 50% aqueouspyridine and 80% acetic acid. 2′-O-Benzoyluridine 3′-phosphoro-β-naphthylidate was used for synthesis of dinucleotides.Keywords
This publication has 4 references indexed in Scilit:
- Transfer ribonucleic acids and related compounds. VI. Synthesis of yeast alanine transfer ribonucleic acid 3'-terminal nonanucleotides and 5'-terminal hexanucleotidesJournal of the American Chemical Society, 1973
- Polynucleotides. XCVIII. A convenient and general method for the preparation of protected dideoxyribo nucleotides containing 5'-phosphate end groupsJournal of the American Chemical Society, 1971
- Synthesis of protected ribooligonucleotides with 3'-phosphate end groupsJournal of the American Chemical Society, 1969
- The estimation of phosphorusBiochemical Journal, 1940