Evaluation of a selective enrichment technique for the isolation of Campylobacter pylori

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Abstract
To cultivate Campylobacter pylori from contaminated biopsy specimens, Brucella broth was supplemented with 10% fetal calf serum, 1% Vitox, 1000 units/ml polymyxin B sulfate, 10 μg/ml vancomycin, and 2 μg/ml amphotericin B. Pseudomonas aeruginosa, Candida albicans , and Enterococcus fecalis were cocultivated with C. pylori . All four strains of C. pylori were recoverable at 24 h. When 21 C. pylori strains were studied in pure culture, 86% grew in the selective enrichment medium. In a clinical study, the selective enrichment technique resulted in isolation of C. pylori from 50% of patient samples, compared with isolation from only 36% of samples with agar cultivation. The selectiove enrichment technique may be more sensitive than techniques currently employed to isolate C. pylori from gastric tissue.

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