Model based analysis of real-time PCR data from DNA binding dye protocols

Open Access

9 March 2007

journal article
research article
Published by Springer Nature in BMC Bioinformatics

Vol. 8 (1) , 85
https://doi.org/10.1186/1471-2105-8-85

Abstract

Reverse transcription followed by real-time PCR is widely used for quantification of specific mRNA, and with the use of double-stranded DNA binding dyes it is becoming a standard for microarray data validation. Despite the kinetic information generated by real-time PCR, most popular analysis methods assume constant amplification efficiency among samples, introducing strong biases when amplification efficiencies are not the same.

Keywords

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