Type C Virus Induced by Iododeoxyuridine in the Human Embryonic Cell Strain HEL-12

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Abstract
The induction of a type C virus from a strain of human embryonic lung cells (HEL-12) by iododeoxyuridine (IdUrd) was examined at various times during in vitro propagation. IdUrd did not elicit type C virus production immediately following initiation of cultures from frozen primary HEL-12 cells. After overnight treatment with 30 .mu.g/ml IdUrd the cells expressed viral antigens and produced a type C virus between the 25th-80th day of in vitro growth. Production of the induced type C virus was transient. Single-cell clones of the parental HEL-12 strain were likewise susceptible to type C virus production by IdUrd. The ability of IdUrd to induce virus terminated with the onset of spontaneous type C virus production from HEL-12 cells at 80-120 days of in vitro growth.

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