Appearance of an arachidonic acid 15‐lipoxygenase pathway upon differentiation of the human promyelocytic cell‐line HL‐60

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Abstract
The metabolism of arachidonic acid and 15‐HPETE was studied in a human promyelocytic cell line (HL‐60). Upon exposure to DMSO, HL‐60 cells undergo differentiation and acquire a 15‐lipoxygenase activity while undifferentiated cells challenged with either arachidonic acid or 15‐HPETE did not enzymatically transform these precursors. Products of the arachidonic acid 15‐lipoxygenase pathway were identified by HPLC. UV‐absorption and gas chromatography‐mass spectrometry. Results indicate that upon differentiation HL‐60 cells express a 15‐lipoxygenase activity as well as the ability to transform 15‐HPETE to 8,15‐DHETEs and 14,15‐DHETE. Moreover, these findings suggest that products of the 15‐lipoxygenase cascade may be generated by a single cell system.

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