A plasma-membrane associated ATPase from the acidophilic bacteriu Acidiphilium cryptum
- 30 September 1990
- journal article
- research article
- Published by Canadian Science Publishing in Biochemistry and Cell Biology
- Vol. 68 (10) , 1222-1225
- https://doi.org/10.1139/o90-181
Abstract
A membrane-bound ATPase of Acidiphilium cryptum, an acidophilic bacterium of mine origin, has been studied. The enzyme has a pH optimum of 8.4. Mg2+ is required for its activity and could be replaced by Mn2+, but not by Ca2+. The enzyme shows a strong preference for ATP as substrate, with the apparent Km of about 0.2 mM. Sulphite ion significantly stimulated the enzyme activity. N,N''-Dicyclohexylcarbodiimide, oligomycin, and azide strongly inhibited the enzyme, whereas vanadate was without effect, suggesting that the A. cryptum ATPase might be of F0F1 type.This publication has 9 references indexed in Scilit:
- The high‐affinity K+‐translocating ATPase complex from Bacillus acidocaldarius consists of three subunitsMolecular Microbiology, 1989
- Acidiphilium organovorum sp. nov., an Acidophilic Heterotroph Isolated from a Thiobacillus ferrooxidans CultureInternational Journal of Systematic and Evolutionary Microbiology, 1986
- Acidiphilium angustum sp. nov., Acidiphilium facilis sp. nov., and Acidiphilium rubrum sp. nov.: Acidophilic Heterotrophic Bacteria Isolated from Acidic Coal Mine DrainageInternational Journal of Systematic and Evolutionary Microbiology, 1986
- Membrane-bound ATPase of a thermoacidophilic archaebacterium, Sulfolobus acidocaldariusBiochimica et Biophysica Acta (BBA) - Biomembranes, 1985
- Activation studies by phospholipids on the purified cytochromec 4:o oxidase ofAzotobacter vinelandiiJournal of Bioenergetics and Biomembranes, 1984
- Acidiphilium cryptum gen. nov., sp. nov., Heterotrophic Bacterium From Acidic Mineral EnvironmentsInternational Journal of Systematic and Evolutionary Microbiology, 1981
- Chapter III Chemical Analysis of Microbial CellsPublished by Elsevier ,1971
- Studies on the Sulfite-dependent ATPase of a Sulfur Oxidizing Bacterium, Thiobacillus thiooxidansThe Journal of Biochemistry, 1967
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951