Facilitated Forward Chemical Genetics Using a Tagged Triazine Library and Zebrafish Embryo Screening
- 9 September 2003
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 125 (39) , 11804-11805
- https://doi.org/10.1021/ja035334d
Abstract
An improved forward chemical genetics approach was successfully demonstrated using a tagged library concept. A small-molecule triazine library with linkers was used to screen for brain/eye developmental phenotypes in a zebrafish embryo system. This approach enabled the rapid isolation of the target proteins by facile affinity matrix preparation and elucidated the first small-molecule inhibitors for several ribosomal accessory proteins or their complex as the target.Keywords
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