Investigations on Growth and Metabolism of Plant Cells

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Abstract
This paper describes a technique for comparisons of the metabolism of rapidly growing and non-growing tissues. The requisite conditions for such studies are discussed. Active tissue growth is obtained by using aseptic conditions and a complete nutrient medium with coco-nut milk added. Rapid diffusion and gas exchange is obtained by using special tubes in which the tissue and medium are rotated in the presence of a large volume of air. Modifications are described which enable various types of metabolic treatment to be used, including the use of tracer carbon. The tissue principally described is carrot phloem, but various other possibilities including the use of micro-organisms are envisaged.

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