Relationship Between ATP Content and Post Thaw Motility in Bull Semen

Abstract
The ATP content in frozen and thawed semen from 17 bulls was determined by a bioluminescence method. The post thaw motility was assessed by phase contrast microscopy by subjective estimation of the percentage of sperm cells with forward motility. The concentration of spermatozoa was counted in a Bürker chamber. The correlation between ATP content and the number of sperm cells with forward motility was high.