Label-free detection of microarrays of biomolecules by oblique-incidence reflectivity difference microscopy

15 March 2004

journal article
Published by Optica Publishing Group in Optics Letters

Vol. 29 (6) , 581-583
https://doi.org/10.1364/ol.29.000581

Abstract

We developed an oblique-incidence reflectivity difference (OI-RD) scanning microscope for label-free imaging of microarrays of biomolecules upon solid substrates. We demonstrate that hybridization reactions in an oligonucleotide microarray fabricated upon a glass slide can be detected by such an OI-RD microscope.

Keywords

This publication has 11 references indexed in Scilit:

Protein microarrays and proteomics
Nature Genetics, 2002
A perspective on protein microarrays
Nature Biotechnology, 2002
Laser pointing stability measured by an oblique-incidence optical transmittance difference technique
Review of Scientific Instruments, 2001
Oligonucleotides form a duplex with non-helical properties on a positively charged surface
Nucleic Acids Research, 2001
Surface Plasmon Resonance Imaging Measurements of Ultrathin Organic Films
Annual Review of Physical Chemistry, 2000
Correlation between Protein and mRNA Abundance in Yeast
Molecular and Cellular Biology, 1999
[12] DNA arrays for analysis of gene expression
Published by Elsevier ,1999
Epitaxial growth of ${SrTiO}_{3}$ on ${SrTiO}_{3} (001)$ using an oblique-incidence reflectance-difference technique
Physical Review B, 1998
Ellipsometric and Interferometric Characterization of DNA Probes Immobilized on a Combinatorial Array
Langmuir, 1997
An optical differential reflectance study of adsorption and desorption of xenon and deuterium on Ni(111)
Applied Physics A, 1996