Liquid-chromatographic analysis for methylphenidate (Ritalin) in serum.
Open Access
- 1 March 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 25 (3) , 401-404
- https://doi.org/10.1093/clinchem/25.3.401
Abstract
We describe a "high performance" liquid chromatographic method for quantitating methylphenidate in serum. The internal standard, 4,5-diphenylimidazole, and serum or plasma sample are extracted in chloroform, evaporated, and redissolved in 20 mmol/L potassium phosphate (pH 3.5)/high-purity acetonitrile, 80/20 by vol. A centrifuged aliquot is chromatographed on mu-Bondapak C-18 with the phosphate/acetonitrile solvent as mobile phase, a flow rate of 1.6 mL/min, and a column temperature of 40 degrees C. Absorbances are read at 192 nm. This method reliably measures concentrations greater than 20 micrograms/L and has analytical recoveries of 74%.This publication has 4 references indexed in Scilit:
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- Pharmacokinetics of Methylphenidate in the Rat Using Single-Ion Monitoring GLC-Mass SpectrometryJournal of Pharmaceutical Sciences, 1977
- Stimulant-Related State-Dependent Learning in Hyperactive ChildrenScience, 1976
- Distribution and elimination of methylphenidate-C14Toxicology and Applied Pharmacology, 1960