Localization by fluorescence in situ hybridization (FISH) of human mitochondrial polymerase γ (POLG) to human chromosome band 15q24→q26, and of mouse mitochondrial polymerase γ (Polg) to mouse chromosome band 7E, with confirmation by direct sequence analysis of bacterial artificial chromosomes (BACs)
Open Access
- 1 January 1997
- journal article
- research article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 78 (3-4) , 281-284
- https://doi.org/10.1159/000134672
Abstract
Cloned cDNAs for the human mitochondrial DNA polymerase γ (POLG) were identified by homology with the yeast mitochondrial DNA polymerase catalytic subunit (MIP). Fluorescence in situ hybridization (FISH) of human and mouse bacterial artificial chromosomes (BACs), hybridized by radioactively labeled POLG cDNAs, mapped to human chromosome band 15q24→q26, as well as to mouse chromosome band 7E. Direct sequencing of the BAC DNA without subcloning confirmed the presence of both human POLG and mouse mitochondrial DNA polymerase γ (Polg) in the respective BACs.Keywords
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