Isolation of the major intrinsic transmembrane protein of the human erythrocyte membrane
- 1 December 1976
- journal article
- Published by Springer Nature in The Journal of Membrane Biology
- Vol. 26 (1) , 173-187
- https://doi.org/10.1007/bf01868872
Abstract
The major intrinsic protein of the human erythrocyte membrane commonly referred to as “Band 3”, was isolated by a multi-step procedure. Extraction of ghost membranes in dilute solutions of lithium diiodosalicylate removed most of the proteins considered to be extrinsic to the membrane. The resulting membrane fragments were solubilized in sodium dodecyl sulfate, and the major sialoglycoprotein (glycophorin A) was removed by wheat germ agglutinin-Sepharose affinity chromatography. Gel filtration in sodium dodecyl sulfate was used as the final step to yield the band 3 polypeptide in electrophoretically homogeneous form.Keywords
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