A FLUORESCENT METHOD FOR THE DETECTION AND LOCALIZATION OF ZINC IN HUMAN GRANULOCYTES

1 November 1969

journal article
Published by SAGE Publications in Journal of Histochemistry & Cytochemistry

Vol. 17 (11) , 749-750
https://doi.org/10.1177/17.11.749

Abstract

A new cytochemical method is presented for the fluorescent demonstration of zinc in human granulocytes using 8-hydroxyquinoline. This compound is known to form fluorescent complexes with magnesium, calcium and zinc. Specificity for zinc is accomplished by adjusting the staining solution to pH 8.0 with Michaelis universal buffer. The technique is simple, rapid and much more specific for zinc than the dithizone stain.

Keywords

BUFFER
FLUORESCENT
COMPOUND
DITHIZONE
HYDROXYQUINOLINE
CYTOCHEMICAL
ACCOMPLISHED
MICHAELIS
ZINC IN HUMAN

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