Gasometric Determination of Hydrogen Peroxide

Abstract
Detns. of the peroxide content of irradiated broth using catalase in a Warburg respirometer were found to be inaccurate. In the presence of excess catalase the oxygen evolved approaches a value of twice that indicated by the accepted general equation for enzymatic decomposition of H2O2; if too little enzyme was used the reaction was slow and incomplete. The method was unsatisfactory for the quantitative detn. of peroxide.
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