Investigations of tissue folates in normal and malignant tissues
- 28 February 1983
- journal article
- research article
- Published by Springer Nature in British Journal of Cancer
- Vol. 47 (3) , 393-398
- https://doi.org/10.1038/bjc.1983.59
Abstract
The folates present in liver, gut and tumour tissue were examined before and after autolysis. Before autolysis 10-formylfolate tetraglutamate (10-CHOFA(glu)4), 5-methyltetrahydrofolate triglutamate (5-CH3THF(glu)3) and possibly tetrahydrofolate polyglutamate(s) (THF(glu)n) were detected. Liver contained all 3 species whereas no 5-CH3THF(glu)3 was present in the tumours, gut showed an intermediate situation. After autolysis the predominant monoglutamates formed were 5-CH3THF in the liver, 10-formylfolates in the gut and possibly tetrahydrofolate (THF) in the tumour extracts. These differences illustrate changes in tissue folates with the proliferation rate of the tissue and suggest an explanation for the methionine auxotrophy of Walker 256 carcinosarcoma cells.Keywords
This publication has 20 references indexed in Scilit:
- High in vivo rates of methionine biosynthesis in transformed human and malignant rat cells auxotrophic for methionine.Proceedings of the National Academy of Sciences, 1976
- Cyclic variations in folate composition and pteroylpolyglutamyl hydrolase (conjugase) activity of the rat uterusThe American Journal of Clinical Nutrition, 1976
- Folate metabolism in the rat liver during regeneration after partial hepatectomyBiochemical Journal, 1975
- Polyglutamate Forms of Folate in Resting and Proliferating Mammalian TissuesClinical Science, 1975
- The Effect of Replacement of Methionine by Homocystine on Survival of Malignant and Normal Adult Mammalian Cells in CultureProceedings of the National Academy of Sciences, 1974
- Reciprocal changes in the levels of functionally related folate enzymes during the culture cycle in human fibroblastsBiochemical and Biophysical Research Communications, 1973
- The enzymology of the formation and interconversion of labile 1-carbon groups in five hepatomas and in Walker tumor 256.1972
- The synthesis of biologically active pteroyloligo- 0L-glutamates (folic acid conjugates). Evaluation of ( 3 H)pteroylheptaglutamate for metabolic studies.1972
- Identification of folic acid compounds in rat liverBiochemical and Biophysical Research Communications, 1972
- A convenient method for the preparation of dl-5-Methyltetrahydrofolic acid (dl-5-methyl-5,6,7,8-tetrahydropteroyl-l-monoglutamic acid)Analytical Biochemistry, 1970