Limitations of fluorescent antibody staining ofHistoplasma capsulatumin tissue sections
- 1 January 1972
- journal article
- Published by Oxford University Press (OUP) in Medical Mycology
- Vol. 10 (2) , 157-163
- https://doi.org/10.1080/00362177285190311
Abstract
Unadsorbed as well as adsorbed fluorescein-labeled Histoplasma capsulatum antiglobulins stain H. capsulatum in culture and in paraffin sections of fixed tissue from active cases of histoplasmosis. Hence, these reagents can be used to great advantage for the identification of the organism in active histoplasmosis. However, in calcified (healed) lesions, fluorescent antibody staining becomes unreliable and is inferior to comparatively simple methods like the use of silver methenamine stain of Grocott.Keywords
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