Small nuclear RNAs in the ciliateTetrahymena
Open Access
- 11 June 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 13 (11) , 4203-4224
- https://doi.org/10.1093/nar/13.11.4203
Abstract
We have isolated and partially characterized a family of small nuclear RNAs (snRNAs) from three different species of the protozoan Tetrahymena. We find six distinct snRNAs ranging in size from 100 to 250 nucleotides. The two largest snRNAs, as well as an abundant, heterogenous group of smaller snRNAs are found in the nucleolar RNA fraction. None of the snRNAs are transcription products of the ribosomal RNA gene or its flanking regions, as shown by hybridization tests. The snRNAs are metabolically stable as determined by pulse/ chase experiments and several of them contain a number of modified nuclotides. The snRNAs from Tetra-hymena all have slightly different sizes from mammalian snRNAs. The cap structure of the snRNAs from Tetrahymena differs from that of the snRNAs from mammalian cells, but has not yet been fully characterized. The relative amount of snRNAs to total RNA is less in Tetrahymena (>0.1%) than in mammalian cells (0.2%)Keywords
This publication has 45 references indexed in Scilit:
- Ribonucleoprotein organization of eukaryotic RNAJournal of Molecular Biology, 1984
- Yeast contains small nuclear RNAs encoded by single copy genesCell, 1983
- Isolation and characterization of an RNA-containing nuclear matrix from Tetrahymena macronucleiBiochemistry, 1979
- Metabolism of low molecular weight ribonucleic acids in early sea urchin embryosBiochemistry, 1979
- The differential inhibitory effect of α‐amanitin on the synthesis of low molecular weight RNA components in BHK cellsFEBS Letters, 1978
- Isolation of the ribosomal RNA gene from Tetrahymena in the state of transcriptionally active chromatinBiochemical and Biophysical Research Communications, 1976
- Translation in vitro of vesicular stomatitis virus mRNA lacking 5′-terminal 7-methylguanosineNature, 1976
- Electrophoretic characterization of shuttling and nonshuttling small nuclear RNAsCell, 1974
- Characterization of rapidly labeled RNA in Tetrahymena pyriformisExperimental Cell Research, 1971
- Low molecular weight RNA associated with 28 s nucleolar RNAJournal of Molecular Biology, 1970