Isolation, Purification and Quantitation of Several Growth Regulating Substances in Ascophyllum nodosum (Phaeophyta)

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Abstract
Two extraction techniques, ethyl acetate and methanol, were used to isolate, purify and quantitate several purine bases, their ribosides, some free cytokinins and other plant hormones including IAA and abscisic acid (ABA) in crude aqueous solutions of A. nodosum. Extraction with ethyl acetate from an aqueous solution at pH 7.7 resulted in almost complete recovery of many of the cytokinins, purines, purine ribosides and ABA. Recovery of IAA was greater using the methanol extraction technique. Following extraction and filtration, GLC recoveries were 65% for ABA, 80% for adenine, and 60% for IAA; this indicates suitability of the described methods for GLC determinations of these plant growth-regulating substances in crude aqueous solutions of the brown alga, A. nodosum.

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