Transcription activation of a UV‐inducible Clostridium perfringens bacteriocin gene by a novel σ factor
Open Access
- 12 January 2005
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 55 (4) , 1196-1206
- https://doi.org/10.1111/j.1365-2958.2004.04456.x
Abstract
Expression of the plasmid-encoded Clostridium perfringens gene for bacteriocin BCN5 was shown to depend in vivo and in vitro on the activity of UviA protein. UviA, also plasmid-encoded, proved to be an RNA polymerase σ factor and was also partly autoregulatory. The uviA gene has two promoters; one provided a UviA-independent, basal level of gene expression while the stronger, UviA-dependent promoter was only utilized after the cell experienced DNA damage. As a result, BCN5 synthesis is induced by treatment with UV light or mitomycin C. UviA is related to a special class of σ factors found to date only in Clostridium species and responsible for activating transcription of toxin genes in Clostridium difficile, Clostridium tetani, and Clostridium botulinum.Keywords
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