Purification, Characterization and Immunological Properties of the Capsular Polysaccharide of Pasteurella haemolytica Serotype T15: Its Identity with the K62 (K2ab) Capsular Polysaccharide of Escherichia coli and the Capsular Polysaccharide of Neisseria meningitidis Serogroup H

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Abstract
Capsular polysaccharide from 2 strains of P. haemolytica serotype T15 was purified and characterized by chemical analysis and NMR spectroscopy. The polymer, a teichoic acid, proved to be very similar in structure to the capsular polysaccharide of P. haemolytica serotype T4 and identical to the previously described K62 (K2ab) capsular polysaccharide of E. coli, and the capsular polysaccharide of N. meningitidis serotype H, i.e, .fwdarw. (2-glycerol-3) .fwdarw. (phosphate) .fwdarw. (4-.alpha.-D-galactopyranose-1) .fwdarw. with partial O-acetylation on the galactose residues. EM with Protein A-gold labeled antisera showed that the polysaccharide was peripherally located on the surface of all 3 organisms. Chemical removal of O-acetyl groups from the polysaccharide yielded a structure identical to that previously described for E. coli K2 (K2a). Both O-acetylated and de-O-acetylated P. haemolytica T15 polymers, when absorbed on to sheep erythrocytes in passive hemagglutination assays, yielded identical antibody titers with sera raised against P. haemolytica T15, E. coli K2 or N. meningitidis H whole cells. De-O-acetylation of the Pasteurella polysaccharide influenced its precipitability with immune sera, but this could not be related to the abesnce of O-acetyl groups because the non-acetylated E. coli K-2 polymer readily precipitated with a line of identity with the acetylated P. haemolytica T15 polymer.

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