An Efficient Technique for Inducing Profuse Sporulation ofAlternariaSpecies

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Abstract
A new technique was developed for inducing profuse sporulation of A. solani [early blight pathogen of potato, Solanum tuberosum], A. alternata and A. dauci in pure culture without the concomitant production of aerial mycelium. Agar blocks from cultures on primary media were transferred to the surface of a 2nd (sporulation) medium of water agar plus CaCO3 and incubated in the dark at 18.degree. C. Spores were harvested after 18-24 h. Initial addition of sucrose to the sporulation medium allowed repeated harvest of conidia at 24 h intervals. Helminthosporium maydis race T also sporulated well when subjected to this protocol, but A. zinniae did not.

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