Fluorescent pH Indicator. Spectral Changes of 4-Methylumbelliferone
- 1 January 1968
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 1 (7) , 423-428
- https://doi.org/10.1080/00032716808051147
Abstract
4-Methylumbelliferone is a useful acid-base fluorescent indicator with a midpoint of its transition interval at pH 7.6. Both anionic and neutral forms fluoresce in the blue with high quantum yield (70%). The indicator properties are based on a blue shift of the absorption spectrum in acid. Depending on the excitation wavelength employed, one has the option of following either an increase or a decrease in fluorescence as the pH changes.Keywords
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