Effectiveness of Ruelene Applied as Localized “Pour-On”and as Spray for Cattle Grub Control1

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Abstract
Ruelene® (4-tert-butyl-2-chlorophenyl methyl methylphos-phoramidate), formulated as a 4 lb./gal. emulsifiable concentrate, was applied to cattle for the control of cattle grubs, Hypoderma lineatum (De Vill.) and H. bovis (L.). Applications were made by high pressure spray at 0.25% concentration, and by a localized “pour-on” technique (details of which are described) utilizing, in the definitive tests, a long-handled dipper to discharge 100 ml. of concentrated emulsion on the back of each test animal. Direct comparison was made of a thorough-coverage spray, at 0.25%, with a“pour-on” application consisting of 20 ml. of the 4 Ib./gal. E.C. in 100 ml./head (equivalent to 1 gal. 0.25% spray-mix or 9.6 gm. actual Ruelene). The spray gave 80% and the “pour-on” gave 99% control (8 calves/treatment, 25.4 grubs/head in untreated controls). Equivalent but slight erythrocyte cholinesterase depression was detected in both treated groups. Slight weight gain differentials favored the treated groups but without significance. A group of 49 calves similarly treated by the “pour-on” technique demonstrated 96% to 98% reduction as compared with 45 well-infested, untreated controls. In another “pour-on” test 14 calves treated at a 10 ml. dosage showed 85% to 88% reduction; another 14 calves treated at a 20-ml. dosage showed 98% reduction; and 9 calves treated at a 40-ml. dosage showed 100% reduction in the number of grubs present as compared with 11 untreated controls. Some mild skin irritation was seen in some heavily dosed dairy eattle, but none occurred on any of the Hereford cattle treated in these tests.

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