Multiple potential regulatory elements in the 5′ flanking region of the β-adrenergic receptor

Abstract
Since the promoter regions of several genes which modulate intracellular cAMP contain cAMP responsive elements which participate in the regulation of transcription, we assessed whether similar elements were present in the 5′ flanking region of the recently cloned human β-adrenergic receptor. This receptor is coupled to cAMP formation, and may play a key role in adrenergic receptor-mediated lipolysis and thermogenesis. Therefore, from a human genomic library we cloned the β3-AR coding block and a 1,320 base pair region immediately 5′ upstream from the initiation of translation. Based on the classic consensus sequence, we found four potential cAMP responsive elements in this 5′ flanking region: CGAGGTCA, TCACATCA, TGAGGTCT and TGACTCCA.