Hormonal Control of Uterine Growth: Alterations in Luminal Epithelial Deoxyribonucleic Acid Synthesis after Intraluminal Application of Estrogen*

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Abstract
Administration of a single injection of estradiol (E2) causes a maximum increase in DNA synthesis in the [rat] uterine luminal epithelium approximately 24 h later. Animals receiving a 2nd injection of E2 15-18 h after the 1st showed an apparent decrease in DNA synthesis measured in this cell type at 24 h. This apparent decrease in DNA synthesis is due to a shift in the time course of DNA synthesis rather than an absolute decrease in this parameter. This inhibitory effect of a 2nd injection of E2 was also observed after intraluminal instillation of the hormone. This effect is dose dependent and appears to result from a shift in the time course of luminal epithelial DNA synthesis. The intraluminal instillation of E2 produces the same pattern of nuclear receptor localization as s.c. administration of the hormone. This inhibitory effect previously described apparently results primarily from a direct action of E2 on the luminal epithelium.