Purification of recombinant proteins from mammalian cell culture using a generic double-affinity chromatography scheme
- 1 March 2005
- journal article
- Published by Elsevier in Protein Expression and Purification
- Vol. 40 (1) , 77-85
- https://doi.org/10.1016/j.pep.2004.10.023
Abstract
No abstract availableKeywords
This publication has 29 references indexed in Scilit:
- Large‐scale transient transfection of serum‐free suspension‐growing HEK293 EBNA1 cells: Peptone additives improve cell growth and transfection efficiencyBiotechnology & Bioengineering, 2003
- High throughput protein production for functional proteomicsTrends in Biotechnology, 2003
- Versatile Expression System for Rapid and Stable Production of Recombinant ProteinsBiotechnology Progress, 2003
- An engineered IN-1 Fab fragment with improved affinity for the Nogo-A axonal growth inhibitor permits immunochemical detection and shows enhanced neutralizing activityProtein Engineering, Design and Selection, 2002
- Stable, Genetically Engineered F(ab′)2Fragments of Chimeric TNT-3 Expressed in Mammalian CellsHybridoma and Hybridomics, 2002
- Intersubunit Interactions in Plasmodium falciparumThioredoxin ReductaseJournal of Biological Chemistry, 2000
- Natural poly-histidine affinity tag for purification of recombinant proteins on cobalt(II)-carboxymethylaspartate crosslinked agaroseJournal of Chromatography A, 1999
- Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate AdsorbentNature Biotechnology, 1988
- New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residuesJournal of Chromatography A, 1987
- Metal chelate affinity chromatography, a new approach to protein fractionationNature, 1975