In Situ Visualization of DNA Double-Strand Break Repair in Human Fibroblasts
- 24 April 1998
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 280 (5363) , 590-592
- https://doi.org/10.1126/science.280.5363.590
Abstract
A method was developed to examine DNA repair within the intact cell. Ultrasoft x-rays were used to induce DNA double-strand breaks (DSBs) in defined subnuclear volumes of human fibroblasts and DNA repair was visualized at those sites. The DSBs remained in a fixed position during the initial stages of DNA repair, and the DSB repair protein hMre11 migrated to the sites of damage within 30 minutes. In contrast, hRad51, a human RecA homolog, did not localize at sites of DNA damage, a finding consistent with the distinct roles of these proteins in DNA repair.Keywords
This publication has 37 references indexed in Scilit:
- Recombination and joining: different means to the same endsGenes and Function, 1997
- Expression of the human RAD51 gene during the cell cycle in primary human peripheral blood lymphocytesBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1996
- The sbcC and sbcD genes of Escherichia coli encode a nuclease involved in palindrome inviability and genetic recombinationGenes to Cells, 1996
- Parallelizing compilersACM Computing Surveys, 1996
- Cell cycle-dependent expression of the mouseMolecular Genetics and Genomics, 1996
- Menage á trois: Double strand break repair, V(D)J recombination and DNA‐PKBioEssays, 1995
- Structural and functional similarities between the SbcCD proteins of Escherichia coli and the RAD50 and MRE11 (RAD32) recombination and repair proteins of yeastMolecular Microbiology, 1995
- Determination of Radiation-induced DNA Strand Breaks in Individual Cells by Non-radioactive Labelling of 3′ OH EndsInternational Journal of Radiation Biology, 1995
- X-Ray—Induced Breakage and Rejoining of Human Interphase ChromosomesScience, 1983
- Inactivation and Mutation of Cultured Mammalian Cells by Aluminium Characteristic Ultrasoft X-raysInternational Journal of Radiation Biology, 1977