Rat Brain α‐Mannosidase: Purification, Properties, and Interaction with Its Antibodies
- 1 December 1982
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 39 (6) , 1601-1606
- https://doi.org/10.1111/j.1471-4159.1982.tb07993.x
Abstract
.alpha.-D-Mannosidase (EC 3.2.1.24) was purified to homogeneity from adult rat brain. The enzyme, of apparent MW 397,000, appears to be formed of subunits of MW 120,000 made of 2 protomers (62,000) bound by disulfide bridges. Isoelectric focusing gives 2 bands, of pI [isoelectric point] 5.40 and 5.15. Both isoenzymes seem to have the same pH curve (a small peak of activity at pH 4.5 and a maximum of activity around pH 6.0). These 2 isoenzymes are immunologically related.Keywords
This publication has 16 references indexed in Scilit:
- Transient Concanavalin A‐Binding Glycoproteins of the Parallel Fibres of the Developing Rat Cerebellum: Evidence for the Destruction of Their GlycansJournal of Neurochemistry, 1980
- Glycosidases and Cerebellar Ontogenesis in the RatJournal of Neurochemistry, 1980
- A continuous‐flow isoelectric focusing system for protein fractionation: Its description and application in the partial purification of choline acetyltransferaseElectrophoresis, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Histochemical localization of glycosidases in dog epididymisJournal of Molecular Histology, 1979
- α-Mannosidase in human red cellsBiochimica et Biophysica Acta (BBA) - Enzymology, 1979
- Postnatal development of rat cerebellum: Massive and transient accumulation of concanavalin a binding glycoproteins in parallel fiber axolemmaBrain Research, 1978
- Isoelectric focusing in layers of granulated gels II. Preparative isoelectric focusingBiochimica et Biophysica Acta (BBA) - Protein Structure, 1975
- SIALOGLYCOPROTEINS AND SEVERAL GLYCOSIDASES IN DEVELOPING RAT BRAINJournal of Neurochemistry, 1970
- THF EARLY STAGES OF ABSORPTION OF INJECTED HORSERADISH PEROXIDASE IN THE PROXIMAL TUBULES OF MOUSE KIDNEY: ULTRASTRUCTURAL CYTOCHEMISTRY BY A NEW TECHNIQUEJournal of Histochemistry & Cytochemistry, 1966