THE GROWTH OF BACTERIA IN A CHARCOAL-CELLOPHANE SYSTEM

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Abstract
By the combined use of cellophane membranes and solid adsorbents, a method has been devised by which high concns. of micro-organisms can be produced. This system consists of a thin-walled cellophane sac containing the nutrient medium (100 ml.) immersed in a 0.5% NaCl soln. (50 ml.) in which is suspended 0.2% activated charcoal or some other solid adsorbent. Cultivation of micro-organisms in the outer menstruum of this system resulted in yields 2- to 4-fold higher in concn. than were obtained in ordinary broth cultures but there was little difference in total viable cells/culture in the 2 systems. The advantage of the charcoal-cellophane system over ordinary broth cultures was its capacity to produce large numbers of cells in a relatively small volume.