Purification and In Vitro Transcription of a Transfer RNA Gene
Open Access
- 1 September 1971
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 68 (9) , 2268-2272
- https://doi.org/10.1073/pnas.68.9.2268
Abstract
A gene specifying tyrosine transfer RNA has been purified and transcribed in vitro. The purification procedure made use of two specialized transducing phages carrying the tRNA(Tyr) gene of Escherichia coli inserted into their DNA in opposite orientations. The separated heavy strands of the two phages were annealed and the single-stranded tails of the resulting hybrid were removed by digestion with Neurospora endonuclease. The size of the purified double-stranded structures was determined by electron microscopy. These isolated duplexes served as template for the in vitro transcription of tRNA(Tyr)-like molecules.Keywords
This publication has 22 references indexed in Scilit:
- A New Method for the Large Scale Purification of Escherichia coli Deoxyribonucleic Acid-dependent Ribonucleic Acid PolymeraseJournal of Biological Chemistry, 1969
- Isolation of Pure lac Operon DNA*Nature, 1969
- An attempt to concentrate ribosomal RNA cistrons in Bacillus subtilis by Millipore filtrationBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
- PARTIAL PURIFICATION OF NATIVE RRNA AND TRNA CISTRONS FROM Mycoplasma SP. (KID)Proceedings of the National Academy of Sciences, 1969
- Isolation of biologically active transfer RNA from transfer RNA-DNA hybridsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
- Isolation and Characterization of Bacterial Ribosomal RNA CistronsBiophysical Journal, 1968
- Specific inhibition by ATP and other properties of an endonuclease of Neurospora crassaCanadian Journal of Biochemistry, 1968
- Suppressor gene Su3+ of E. coli, a structural gene for tyrosine TRNA.Proceedings of the National Academy of Sciences, 1968
- Isolation of the Hybrid Between Ribosomal RNA and DNA of Bacillus subtilisCold Spring Harbor Symposia on Quantitative Biology, 1968
- AN ENDONUCLEASE FROM NEUROSPORA CRASSA SPECIFIC FOR POLYNUCLEOTIDES LACKING AN ORDERED STRUCTURE .I. PURIFICATION AND PROPERTIES OF ENZYME1965