ANEMIA OF CHRONIC RENAL-FAILURE - STUDIES OF EFFECT OF ORGANIC-SOLVENT EXTRACTION OF SERUM

  • 1 January 1978
    • journal article
    • research article
    • Vol. 92  (3) , 363-369
Abstract
CRF [chronic renal failure] serum contains a material(s) which is inhibitory to erythropoiesis in vitro. The chemical nature and mechanism of action of this material are unclear at the present time. NL [normal] serum and CRF serum samples were extracted with 2 organic solvents, chloroform and petroleum ether. The serum samples were studied before and after extraction in a tissue culture system in which dog erythroblasts were stimualted by EP [erythropoietin] to synthesize heme. Before extraction, cells suspended in CRF serum synthesized less heme than cells cultured in NL serum. Extraction of CRF serum with chloroform, but not petroleum ether, resulted in an improvement in its ability to support heme synthesis. Studies of the material extracted by chloroform from CRF serum showed that it was inhibitory to erythropoiesis. The erythropoietic inhibitory substance in uremic [human] serum was soluble in chloroform but not in petroleum ether, suggesting that it is a polar lipid.

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