Cartilage‐staining technique for the examination of unskinned fetal rat specimens previously processed with alizarin red S

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Abstract
A novel staining technique has been devised that permits a cartilage examination of unskinned fetal rats that have been previously processed for skeletal examination with alizarin red S. The procedure consists of rinsing alizarin red S‐stained specimens in distilled water and placing the specimens in a 3% acetic acid solution. A transfer of the stain from bone to adjacent cartilage occurs, producing purple‐stained cartilaginous structures that can be differentiated from still‐discernible bone structures.